ABSTRACT
This study determined the effects of gallium-aluminum-arsenide laser (GaAlAs), gallium-arsenide laser (GaAs) and Dersani® healing ointment on skin wounds in Wistar rats. The parameters analyzed were: type I and III collagen fiber concentrations as well as the rate of wound closure. Five wounds, 12 mm in diameter, were made on the animals backs. The depth of the surgical incision was controlled by removing the epithelial tissue until the dorsal muscular fascia was exposed. The animals were anesthetized with ketamine and xylazine via intraperitoneal injection. The rats were randomly divided into five groups of 6 animals each, according to the treatment received. Group 1 (L4): GaAs laser (4 J/cm²); group 2 (L30): GaAlAs laser (30 J/cm²); group 3 (L60): GaAlAs laser (60 J/cm²); group 4 (D): Dersani® ointment; group 5 (control): 0.9 percent saline. The applications were made daily over a period of 20 days. Tissue fragments were stained with picrosirius to distinguish type I collagen from type III collagen. The collagen fibers were photo-documented and analyzed using the Quantum software based on the primary color spectrum (red, yellow and blue). Significant results for wound closing rate were obtained for group 1 (L4), 7.37 mm/day. The highest concentration of type III collagen fibers was observed in group 2 (L30; 37.80 ± 7.10 percent), which differed from control (29.86 ± 5.15 percent) on the 20th day of treatment. The type I collagen fibers of group 1 (L4; 2.67 ± 2.23 percent) and group 2 (L30; 2.87 ± 2.40 percent) differed significantly from control (1.77 ± 2.97 percent) on the 20th day of the experiment.
Subject(s)
Animals , Male , Rats , Collagen Type I/radiation effects , Collagen Type III/radiation effects , Lasers, Semiconductor/therapeutic use , Skin/radiation effects , Wound Healing/radiation effects , Low-Level Light Therapy/methods , Rats, WistarABSTRACT
Mayaro virus was grown in BHK-21 cells and purified by centrifugation in a potassium-tartrate gradient (5-50%). The electron microscopy analyses of the purified virus showed an homogeneous population of enveloped particles with 69 ñ 2.3 nm in diameter. Three structural virus proteins were identified and designated pl, p2 and p3. Their average molecular weight were p1, 54 KDa; p2, 50 KDa and p3, 34 KDa. In Mayaro virus infected. Aedes albopictus cells and in BHK-21 infected cells we detected six viral proteins, in wich three of them are the structural virus proteins and the other three were products from processing of precursors of viral proteins, whose molecular weights are 62 KDa, 64 KDa and 110 KDa. The 34 KDa protein was the first viral protein sinthesized at 5 hours post-infection in both cell lines studied
Subject(s)
Arboviruses/ultrastructure , Viral ProteinsABSTRACT
the multiplication of Mayaro virus in Aedes albopictus cells was drastically inhibited after incubation at 37-C. The effect of short-term exposure of infected cells to high temperatures (heat shock) produced a preferential translation of the heat shock messengers when compared to the viral mRNAs. When cells were shifted back to 28-C (the optimum growth temperature for Aedes albopictus cells), preferential translation of viral mRNA occurred. Although the infected cells were programmed for preferential translation of viral messengers, the therminal treatment was able to shif the translational machinery towards synthesis of heat shock proteins